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Improve FRAME setup guide (#16)
* Update 00_Cover_Page.md * Update 00_Cover_Page.md * Update 00_Cover_Page.md * Update 01_Safety_and_Compliance.md * Update 01_Safety_and_Compliance.md * Update 00_Cover_Page.md * Update 00_Cover_Page.md * Update 01_Safety_and_Compliance.md * Update 01_Safety_and_Compliance.md * Update 01_Safety_and_Compliance.md * Update 01_Safety_and_Compliance.md * Update 02_Introduction_and_Overview.md * Update 02_Introduction_and_Overview.md * Update 02_Introduction_and_Overview.md * Update 02_Introduction_and_Overview.md * Update 02_Introduction_and_Overview.md * Update 02_Introduction_and_Overview.md * Update 02_Introduction_and_Overview.md * Update 03_Technical_Specifications.md * Update 03_Technical_Specifications.md * Update 03_Technical_Specifications.md * Update 03_Technical_Specifications.md * Update 03_Technical_Specifications.md * Update 04_Installation_and_Setup.md * Update 04_Installation_and_Setup.md * Create Temp * Add files via upload * Update 04_Installation_and_Setup.md * Update 04_Installation_and_Setup.md * Update 04_Installation_and_Setup.md * Update 04_Installation_and_Setup.md * Update 03_Technical_Specifications.md * Update 04_Installation_and_Setup.md * Update 04_Installation_and_Setup.md * Update 04_Installation_and_Setup.md * Update 04_Installation_and_Setup.md * Update 04_Installation_and_Setup.md * Update 04_Installation_and_Setup.md * Update 04_Installation_and_Setup.md * Update 04_Installation_and_Setup.md * Update 04_Installation_and_Setup.md * Update 04_Installation_and_Setup.md * Update 04_Installation_and_Setup.md * Update 04_Installation_and_Setup.md * Update 04_Installation_and_Setup.md * Update 04_Installation_and_Setup.md * Update Optical_Alignment_Procedures.md * Update 04_Installation_and_Setup.md * Update Coordinate_System_Reference.md * Update Coordinate_System_Reference.md * Update Coordinate_System_Reference.md * Update Coordinate_System_Reference.md * Update Coordinate_System_Reference.md * Update 04_Installation_and_Setup.md * Update 04_Installation_and_Setup.md * Update 03_Technical_Specifications.md * Update 05_Operation_Manual.md * Update 04_Installation_and_Setup.md * Update 04_Installation_and_Setup.md * Update 04_Installation_and_Setup.md * Update 04_Installation_and_Setup.md * Update 05_Operation_Manual.md * Update 05_Operation_Manual.md * Update 05_Operation_Manual.md * Update 05_Operation_Manual.md * Update 06_Maintenance_and_Troubleshooting.md * Update 01_Safety_and_Compliance.md * Update 00_Cover_Page.md * Update 03_Technical_Specifications.md * Update 03_Technical_Specifications.md * Update 04_Installation_and_Setup.md * Update 01_Safety_and_Compliance.md * Update 04_Installation_and_Setup.md * Update 04_Installation_and_Setup.md * Update 04_Installation_and_Setup.md * Update 04_Installation_and_Setup.md * Update 04_Installation_and_Setup.md * Update 05_Operation_Manual.md * Update 05_Operation_Manual.md * Create 03a_flowchart_step_by_step.md * Update 03a_flowchart_step_by_step.md * Update 03a_flowchart_step_by_step.md * Update 05_Operation_Manual.md * Update 05_Operation_Manual.md * Update 05_Operation_Manual.md * Update 05_Operation_Manual.md * Update 05_Operation_Manual.md * Update 05_Operation_Manual.md * Update 05_Operation_Manual.md * Update 05_Operation_Manual.md * Update 03a_flowchart_step_by_step.md * Update 00_Cover_Page.md * Update 01_Safety_and_Compliance.md * Update 02_Introduction_and_Overview.md * Update 03_Technical_Specifications.md * Update 03_Technical_Specifications.md * Delete docs/03_Investigator/02_FRAME/03a_flowchart_step_by_step.md * Update 04_Installation_and_Setup.md * Update 05_Operation_Manual.md * Update 05_Operation_Manual.md * Update 05_Operation_Manual.md * Update 06_Maintenance_and_Troubleshooting.md --------- Co-authored-by: Mikrodoktor <[email protected]>
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docs/03_Investigator/02_FRAME/00_Cover_Page.md

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- **IEC 61010-1** (Safety requirements for electrical equipment)
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- **EN 55011** (Electromagnetic compatibility)
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The CE marking indicates conformity with applicable regulations.
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**The CE marking indicates conformity with applicable regulations.**
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---
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## Important Safety Notice
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Hans-Knöll-Str. 6
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07745 Jena, Germany
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**Technical Support**: hello@openuc2.com (openuc2.discourse.group)
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**Technical Support**: support@openuc2.com (openuc2.discourse.group)
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**Sales Information**: [email protected]
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**General Information**: [email protected]
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**Wiki**: https://openuc2.github.io

docs/03_Investigator/02_FRAME/01_Safety_and_Compliance.md

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1. **Moving Parts**: Keep hands and loose clothing away from moving parts
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2. **Sample Handling**: Use appropriate tools when handling samples
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3. **Weight**: Use proper lifting techniques when moving the microscope (~20Kg)
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3. **Weight**: Use proper lifting techniques when moving the microscope (~20kg)
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4. **Sharp Edges**: Be aware of sharp edges on optical components and mounts
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5. **Rapid Prototyping**: Customize optical systems
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### Chemical Safety
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## Emergency Procedures
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### Electrical Emergency
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1. Immediately disconnect power at the main switch
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2. Do not touch exposed electrical components
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3. Contact qualified service personnel
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1. Immediately press emergency stop button
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2. Immediately disconnect power at the main switch
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3. Do not touch exposed electrical components
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4. Contact qualified service personnel
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### Fire Emergency
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1. Disconnect power immediately
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2. Use appropriate fire extinguisher
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3. Evacuate area if necessary
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1. Immediately press emergency stop button
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2. Disconnect power immediately
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3. Use appropriate fire extinguisher
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4. Evacuate area if necessary
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### Chemical Spill
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1. Follow laboratory chemical spill procedures

docs/03_Investigator/02_FRAME/02_Introduction_and_Overview.md

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## What is the FRAME Microscope?
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The **FRAME** (Fast Rigid Automated Microscope Engine) represents a new approach to microscopy: an open, modular, automated, and flexibly adaptable microscope system. Based on the modular optics construction kit UC2 (You.See.Too.), the FRAME system lowers barriers to access complex optical and microscopy technology.
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The **FRAME** (Fast Rigid Automated Microscope Engine) represents a new approach to microscopy: an open, modular, automated, and flexibly adaptable microscope system. Based on UC2's (You.See.Too.) modular optics kit, the FRAME system lowers barriers to access complex optical and microscopy technology.
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## System Philosophy
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The FRAME system is built on complete modularization of all components regarding mechanics, electronics, and software:
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- **Interchangeable Modules**: Cube modules can be freely exchanged to switch between brightfield, fluorescence, or interference microscopy
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- **Flexible Mechanics**: Allows both classical objective setups and free arrangement for special geometries
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- **Flexible Mechanics**: Allows both, classical objective setups and free arrangements for special geometries
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- **Smart Electronics**: Each cube can optionally have its own control unit for motors, galvo mirrors, or LEDs. All components share the same CAN BUS via 12V supply voltage.
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- **Open Interface**: System control via user-friendly graphical interface or programming interface (Python, REST, CAN BUS, USB)
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- **Precision XYZ System**: High-accuracy positioning and focusing mechanisms
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- **Removable Optics Unit**: Modular design allows easy reconfiguration
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- **Slide-In System**: Standardized 50mm cubes with quick-exchange capability
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- **Linear Objective Revolver**: Decide between two different magnifications/resolutions
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- **Linear Objective Revolver**: Switch between two different magnifications/resolutions
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### Electronic Architecture
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### Industrial Applications
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1. **Quality Control**: Materials inspection and defect analysis
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2. **Semiconductor Industry**: Wafer inspection and process control
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3. **Biotechnology**: Process monitoring and product validation
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4. **Education**: Modern imaging technique visualization in teaching and training
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2. **Biotechnology**: Process monitoring and product validation
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3. **Education**: Modern imaging technique visualization in teaching and training
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### Advantages for Different User Groups
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### Open Source Benefits
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- **Global Accessibility**: Electronic platforms can be replicated worldwide, including in resource-limited regions
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- **Knowledge Sharing**: Open documentation promotes global access to high technology
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- **Knowledge Sharing**: Open documentation promotes global access to high-end technology
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- **Community Development**: Active community ensures continuous improvement and support
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- **Local Repairs**: Using 3D printing and digital fabrication, broken parts can be repaired on site
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docs/03_Investigator/02_FRAME/03_Technical_Specifications.md

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![](./IMAGES/02/FRAME_Overview_2.png)
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### Overall System Dimensions
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### Overall System Dimensions (including illumination arm)
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| Parameter | Motorized Version |
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|-----------|-------------------|
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| Width | 250 mm |
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| Depth | 250 mm |
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| Height | 250 mm |
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| Weight | 20 kg |
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| Width | 310 mm |
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| Depth | 410 mm |
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| Height | 400 mm |
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| Weight | 25 kg |
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### Mechanical Specifications
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#### Frame Construction
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- **Material**: Anodized aluminum profiles and plates
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- **Vibration Damping**: Integrated damping system (bumper feed)
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- **Stage**: Integrated XYZ Stage in one mechanical design
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- **Objective Revolver**: 2 Slots for multiple magnifications/resolutions (max diameter <28mm)
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#### XYZ Positioning System
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- **X-Y Travel Range**: up to 120 × 90 mm (Well plate format), stepsize: ~300nm
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- **Z-Travel Range**: 8 mm, stepsize: ~300nm
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- **Resolution**: 0.3 μm (motorized)
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- **Accuracy**: ±XXXX μm
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- **Repeatability**: ±0.5 μm
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- **Objective Revolver**: 2 Slots for multiple magnifications/resolutions
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### Optical Specifications
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#### Camera Interface
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- **Mount Type**: C-mount
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- **Sensor Compatibility**: CMOS
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- **Available Cameras:** HIK IMX 179, IMX275
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- **Available Cameras:** HIK IMX179, IMX275
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- **Connection**: USB 3.0
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- **Trigger**: Hardware and software trigger support
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### Environmental Requirements
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#### Operating Conditions
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- **Temperature**: 5°C to 38°C (59°F to 95°F)
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- **Temperature**: 15°C to 35°C
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- **Humidity**: 30% to 80% relative humidity, non-condensing
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- **Atmospheric Pressure**: 86 kPa to 106 kPa
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- **Altitude**: Up to 2000 m above sea level
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- **Humidity**: 10% to 95% relative humidity, non-condensing
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#### Installation Requirements
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- **Vibration**: Isolated surface recommended for best performance
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- **Vibration**: optical table with vibration damping recommended for best performance
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- **Space**: 1 m × 1.5 m minimum work area
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- **Power**: 230V AC, 50/60 Hz (or 115V AC with adapter)
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- **Ventilation**: Natural convection (no forced ventilation required)
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### Inner Cube System
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#### Cube Drawer Mechanism
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- **Number of Positions**: 4 cube positions (configurable)
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- **Drawer Travel**: 150mm sliding range per position
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- **Drawer Rails**: Precision linear ball bearing guides
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- **Load Capacity**: 500g per cube position
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- **Positioning Accuracy**: ±0.05mm repeatability
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- **Locking Mechanism**: Spring-loaded detent system
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#### Mechanical Specifications
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- **Dimensions**: 50 × 50 × 50 mm
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- **Material**: Anodized aluminum or 3D-printed PLA/PETG
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- **Material**: ABS / injection molded
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- **Precision**: ±0.1 mm dimensional accuracy
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- **Interface**: Dovetail connection system
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- **Mounting**: Threaded inserts M3 and M4 compatible
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#### Motorized Stage
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- **Travel Range**: 75 × 50 mm (standard), 150 × 100 mm (extended)
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- **Resolution**: 0.1 μm
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#### XYZ Positioning System (Motorized Stage)
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- **X-Y Travel Range**: 75 × 50 mm (standard), up to 120 × 90 mm (extended), stepsize: ~300nm
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- **Z-Travel Range**: 8 mm, stepsize: ~300nm
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- **Resolution**: 0.3 μm (motorized)
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- **Accuracy**: ±XXXX μm
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- **Repeatability**: ±0.5 μm
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- **Speed**: 0.1 μm/s to 10 mm/s
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- **Acceleration**: Programmable, up to 100 mm/s²
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- **Load Capacity**: 2 kg maximum
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- **Resolution**: 0.05 μm
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- **Speed**: 0.1 μm/s to 5 mm/s
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- **Repeatability**: ±0.1 μm
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- **Autofocus**: Laser-based or image-based
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- **Autofocus**: Laser-based or image-based (optional)
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## Compatibility and Standards
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