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fb46143
Added first draft of low-biomass ppl
bnovak32 Sep 8, 2025
60fb1fc
Fixed Assembly section numbering
bnovak32 Sep 8, 2025
5821e1d
Added read-based decontamination
bnovak32 Sep 19, 2025
a332edf
added missing parameter definition
bnovak32 Sep 19, 2025
52abb0c
Adding Low Biomass Nanopore Metagenomics Pipeline document
asaravia-butler Sep 22, 2025
02f19f8
Update Low Biomass pipeline draft (#176)
bnovak32 Sep 30, 2025
6e6924c
added decontamination and visualization steps, and fixed broken links
olabiyi Oct 2, 2025
0e0cf11
fixed broken links
olabiyi Oct 2, 2025
2e4f7f1
fixed broken links
olabiyi Oct 2, 2025
9c150ef
made edits to functions and the overall document
olabiyi Oct 6, 2025
cefd6c2
Merge pull request #178 from olabiyi/DEV_Metagenomics_low_biomass
asaravia-butler Oct 8, 2025
519b987
fixed run_decontam()
olabiyi Oct 9, 2025
ba251b7
Changed how filtered table is generated for kraken and kaiju
olabiyi Oct 9, 2025
069fb14
Changed how filtered table is generated for kraken and kaiju
olabiyi Oct 9, 2025
285abb7
Fixed decontamination_table bug
olabiyi Oct 9, 2025
f843916
Fixed bug with reading and writing species table
olabiyi Oct 10, 2025
b6e709e
R code bug fixes
asaravia-butler Oct 13, 2025
96df97f
Updates to the dev low biomass pipeline document GL-DPPD-7116
asaravia-butler Nov 13, 2025
82a7043
Update GL-DPPD-7110-A_annotations.csv
bnovak32 Nov 3, 2025
a93c9c4
Update humann3.yaml
bnovak32 Nov 4, 2025
9c1b618
Updated assay suffixes
bnovak32 Dec 21, 2025
c886d8d
Updates to Low-biomass pipeline docs
bnovak32 Jan 24, 2026
a196c7a
Updated assembly decontamination and heatmap section
bnovak32 Jan 25, 2026
614c145
Finished Illumina low-biomass pipeline draft
bnovak32 Jan 26, 2026
ef65dd5
Update GL-DPPD-7116.md (#188)
asaravia-butler Jan 26, 2026
661fbac
pipeline document updates through step 8c
bnovak32 Jan 27, 2026
cb75153
Update GL-DPPD-7117
bnovak32 Jan 27, 2026
e7cd701
update get_abundant_features functions docs
bnovak32 Jan 27, 2026
2d7d797
Dev metagenomics low biomass - nanopore updates through step 9c (#190)
asaravia-butler Jan 28, 2026
4b21d2d
Finish renumbering and minor formatting edits
bnovak32 Jan 28, 2026
12305ef
typo fix on GL-DPPD-7117.md
asaravia-butler Jan 28, 2026
3965569
typo fix on GL-DPPD-7117.md
asaravia-butler Jan 28, 2026
3c08da6
formatting updates to GL-DPPD-7116.md
asaravia-butler Jan 29, 2026
341b900
Formatting updates to GL-DPPD-7117.md
asaravia-butler Jan 29, 2026
7366989
Dev metagenomics low biomass bn (#195)
bnovak32 Mar 19, 2026
65ae99c
Add low-biomass README
bnovak32 Mar 19, 2026
4f66432
updated Metagenomiocs Workflow links
bnovak32 Mar 23, 2026
03cbf07
added human functional profiling to long-read pipeline
bnovak32 Mar 23, 2026
19747ec
updated Biyi's team designation
bnovak32 Mar 24, 2026
d13b7ba
Added GL-DPPD-7107-B doc
bnovak32 Mar 25, 2026
1a1ddd9
updated low-biomass README with workflow doc link
bnovak32 Mar 25, 2026
a779e3c
updated header
bnovak32 Mar 25, 2026
5367c7e
Update GL-DPPD-7107-B.md
bnovak32 Mar 26, 2026
468915b
formatting and software version updates
bnovak32 Apr 1, 2026
69b3f0e
updated bbduk to BBTools
bnovak32 Apr 4, 2026
a3ad2d6
Final updates
bnovak32 Apr 15, 2026
e21868f
Updated Metagenomics READMEs and added NF_Metagenomics repository lin…
bnovak32 Apr 15, 2026
a8ca305
Updated Metagenomics workflow name (#199)
bnovak32 Apr 15, 2026
44cce13
Update README.md
bnovak32 Apr 18, 2026
ee00d2b
Update README.md
bnovak32 Apr 18, 2026
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8 changes: 8 additions & 0 deletions .gitmodules
Original file line number Diff line number Diff line change
@@ -1,3 +1,11 @@
[submodule "Amplicon/Illumina/Workflow_Documentation/NF_AmpIllumina"]
path = Amplicon/Illumina/Workflow_Documentation/NF_AmpIllumina
url = https://github.com/nasa/GeneLab_AmpliconSeq_Workflow
[submodule "Metagenomics/Low_Biomass/Workflow_Documentation/NF_MetagenomeSeq"]
path = Metagenomics/Low_Biomass/Workflow_Documentation/NF_MetagenomeSeq
url = https://github.com/nasa/GeneLab_Metagenomics_Workflow/
branch = DEV
[submodule "Metagenomics/Illumina/Workflow_Documentation/NF_MetagenomeSeq"]
path = Metagenomics/Illumina/Workflow_Documentation/NF_MetagenomeSeq
url = https://github.com/nasa/GeneLab_Metagenomics_Workflow/
branch = DEV
Original file line number Diff line number Diff line change
Expand Up @@ -11,7 +11,7 @@ ECOLI,Escherichia coli,str. K-12 substr. MG1655,59,ensembl_bacteria,https://ftp.
HUMAN,Homo sapiens,,112,ensembl,https://ftp.ensembl.org/pub/release-112/fasta/homo_sapiens/dna/Homo_sapiens.GRCh38.dna.primary_assembly.fa.gz,https://ftp.ensembl.org/pub/release-112/gtf/homo_sapiens/Homo_sapiens.GRCh38.112.gtf.gz,9606,org.Hs.eg.db,,https://figshare.com/ndownloader/files/48354445,https://figshare.com/ndownloader/files/48354448
,Lactobacillus acidophilus,NCFM,,ncbi,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/011/985/GCF_000011985.1_ASM1198v1/GCF_000011985.1_ASM1198v1_genomic.fna.gz,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/011/985/GCF_000011985.1_ASM1198v1/GCF_000011985.1_ASM1198v1_genomic.gtf.gz,272621,,,https://figshare.com/ndownloader/files/49061254,https://figshare.com/ndownloader/files/49061257
MOUSE,Mus musculus,,112,ensembl,https://ftp.ensembl.org/pub/release-112/fasta/mus_musculus/dna/Mus_musculus.GRCm39.dna.primary_assembly.fa.gz,https://ftp.ensembl.org/pub/release-112/gtf/mus_musculus/Mus_musculus.GRCm39.112.gtf.gz,10090,org.Mm.eg.db,,https://figshare.com/ndownloader/files/48354460,https://figshare.com/ndownloader/files/48354457
,Mycobacterium marinum,M,,ncbi,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/018/345/GCF_000018345.1_ASM1834v1/GCF_000018345.1_ASM1834v1_genomic.gtf.gz,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/018/345/GCF_000018345.1_ASM1834v1/GCF_000018345.1_ASM1834v1_genomic.gtf.gz,216594,,,https://figshare.com/ndownloader/files/49061260,https://figshare.com/ndownloader/files/49061263
,Mycobacterium marinum,M,,ncbi,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/018/345/GCF_000018345.1_ASM1834v1/GCF_000018345.1_ASM1834v1_genomic.fna.gz,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/018/345/GCF_000018345.1_ASM1834v1/GCF_000018345.1_ASM1834v1_genomic.gtf.gz,216594,,,https://figshare.com/ndownloader/files/49061260,https://figshare.com/ndownloader/files/49061263
ORYSJ,Oryza sativa,Japonica,59,ensembl_plants,https://ftp.ensemblgenomes.ebi.ac.uk/pub/plants/release-59/fasta/oryza_sativa/dna/Oryza_sativa.IRGSP-1.0.dna.toplevel.fa.gz,https://ftp.ensemblgenomes.ebi.ac.uk/pub/plants/release-59/gtf/oryza_sativa/Oryza_sativa.IRGSP-1.0.59.gtf.gz,39947,,,https://figshare.com/ndownloader/files/48354451,https://figshare.com/ndownloader/files/48354454
ORYLA,Oryzias latipes,,112,ensembl,http://ftp.ensembl.org/pub/release-112/fasta/oryzias_latipes/dna/Oryzias_latipes.ASM223467v1.dna.toplevel.fa.gz,http://ftp.ensembl.org/pub/release-112/gtf/oryzias_latipes/Oryzias_latipes.ASM223467v1.112.gtf.gz,8090,,org.Olatipes.eg.db,https://figshare.com/ndownloader/files/48354463,https://figshare.com/ndownloader/files/48354466
,Pseudomonas aeruginosa,UCBPP-PA14,,ncbi,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/014/625/GCF_000014625.1_ASM1462v1/GCF_000014625.1_ASM1462v1_genomic.fna.gz,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/014/625/GCF_000014625.1_ASM1462v1/GCF_000014625.1_ASM1462v1_genomic.gtf.gz,208963,,,https://figshare.com/ndownloader/files/49061266,https://figshare.com/ndownloader/files/49061269
Expand All @@ -21,4 +21,5 @@ SALTY,Salmonella enterica,serovar Typhimurium str. LT2,,ncbi,https://ftp.ncbi.nl
,Serratia liquefaciens,ATCC 27592,,ncbi,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/422/085/GCF_000422085.1_ASM42208v1/GCF_000422085.1_ASM42208v1_genomic.fna.gz,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/422/085/GCF_000422085.1_ASM42208v1/GCF_000422085.1_ASM42208v1_genomic.gtf.gz,1346614,,,https://figshare.com/ndownloader/files/49061278,https://figshare.com/ndownloader/files/49061281
,Staphylococcus aureus,MRSA252,,ncbi,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/011/505/GCF_000011505.1_ASM1150v1/GCF_000011505.1_ASM1150v1_genomic.fna.gz,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/011/505/GCF_000011505.1_ASM1150v1/GCF_000011505.1_ASM1150v1_genomic.gtf.gz,282458,,,https://figshare.com/ndownloader/files/49061284,https://figshare.com/ndownloader/files/49061287
,Streptococcus mutans,UA159,,ncbi,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/007/465/GCF_000007465.2_ASM746v2/GCF_000007465.2_ASM746v2_genomic.fna.gz,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/007/465/GCF_000007465.2_ASM746v2/GCF_000007465.2_ASM746v2_genomic.gtf.gz,210007,,,https://figshare.com/ndownloader/files/49061290,https://figshare.com/ndownloader/files/49061293
,Vibrio fischeri,ES114,,ncbi,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/011/805/GCF_000011805.1_ASM1180v1/GCF_000011805.1_ASM1180v1_genomic.fna.gz,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/011/805/GCF_000011805.1_ASM1180v1/GCF_000011805.1_ASM1180v1_genomic.gtf.gz,312309,,,https://figshare.com/ndownloader/files/49061296,https://figshare.com/ndownloader/files/49061299

,Vibrio fischeri,ES114,,ncbi,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/011/805/GCF_000011805.1_ASM1180v1/GCF_000011805.1_ASM1180v1_genomic.fna.gz,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/011/805/GCF_000011805.1_ASM1180v1/GCF_000011805.1_ASM1180v1_genomic.gtf.gz,312309,,,https://figshare.com/ndownloader/files/49061296,https://figshare.com/ndownloader/files/49061299
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